Human lung Dataset
Created Date: January 2022
This dataset was generated in-house at Resolve Biosciences using customer-provided tissue sections. The experiment used a custom 34-gene target panel to measure gene expression in situ for five samples across three Molecular observation slides (Slide 873, Sample C1; Slide 874, Sample D2; Slide 879, Samples B1, C2, D2). The dataset contains DAPI image, expression matrix, gene list, and cell segmentation files for download. Data made available with kind permission from a Resolve Biosciences customer.
Sample details: Fresh frozen tissue sections of human lung were analyzed to identify hematopoietic cells and progenitor niches.
Files for download:
Molecular observation slide 873
| File Name | Description |
|---|---|
| 00008-slide873_C1_DAPI.tiff | Fluorescence image of the DAPI-stained sample, stitched from individual field of views. |
| 00008-slide873_C1_brightfield.tiff | Brightfield image of the sample, stitched from individual field of views. |
| 00008-slide873_C1_results_withFP.txt | Plain text file containing x, y and z coordinates plus target names of individual RNA molecules as tab separated values. Coordinate values correspond to pixels in the DAPI image. Targets named “FP XX” refer to decoded signals from barcodes not used in the experiment and therefore indicate false positives. |
| 00008-slide873_C1_customer-report.pdf | QC report that provides an overview of various metrics of the assay |
Molecular observation slide 874
| File Name | Description |
|---|---|
| 00008-slide874_D2_DAPI.tiff | Fluorescence image of the DAPI-stained sample, stitched from individual field of views. |
| 00008-slide874_D2_brightfield.tiff | Brightfield image of the sample, stitched from individual field of views. |
| 00008-slide874_D2_results_withFP.txt | Plain text file containing x, y and z coordinates plus target names of individual RNA molecules as tab separated values. Coordinate values correspond to pixels in the DAPI image. Targets named “FP XX” refer to decoded signals from barcodes not used in the experiment and therefore indicate false positives. |
| 00008-slide874_customer-report.pdf | QC report that provides an overview of various metrics of the assay |
Molecular observation slide 879
| File Name | Description |
|---|---|
| 00008-slide879_B1-1_DAPI.tiff | Fluorescence image of the DAPI-stained sample, stitched from individual field of views. |
| 00008-slide879_C2-1_DAPI.tiff | |
| 00008-slide879_D2-1_DAPI.tiff | |
| 00008-slide879_B1-1_brightfield.tiff | Brightfield image of the sample, stitched from individual field of views. |
| 00008-slide879_C2-1_brightfield.tiff | |
| 00008-slide879_D2-1_brightfield.tiff | |
| 00008-slide879_B1-1_results_withFP.txt | Plain text file containing x, y and z coordinates plus target names of individual RNA molecules as tab separated values. Coordinate values correspond to pixels in the DAPI image. Targets named “FP XX” refer to decoded signals from barcodes not used in the experiment and therefore indicate false positives. |
| 00008-slide879_C2-1_results_withFP.txt | |
| 00008-slide879_D2-1_results_withFP.txt | |
| 00008-slide879_customer-report.pdf | QC report that provides an overview of various metrics of the assay |
Gene list
| File Name | Description |
|---|---|
| Resolve_Human_lung_gene_list.txt | List of genes targeted. |
